covid-19, coronavirus, laboratories, sars, sars-cov-2: Studies on SARS-CoV inactivation by chemical and physical agents:

Data on the effectiveness of physical and chemical agents in inactivating SARS-CoV-2 are currently scarce. Based on them, it is difficult to create effective protocols for research and diagnostic laboratories. Due to the significant similarity of both the genome and the structure of SARS-CoV and SARS-CoV-2, we can assume that both viruses react similarly to physical and chemical factors. SARS-CoV scientific research data is extensive and can be helpful in finding effective solutions to the current pandemic.

 

Inactivation of SARS-CoV by gamma radiation:

For viruses, sensitivity to gamma radiation is dependent on the size of the genome. In coronaviruses it is extremely large. SARS-CoV has a genome of 29,751 nucleotides. Experimental data show that 1 megarada of gamma radiation is required to inactivate this virus. SARS-CoV-2 has a genome of 29,811 nucleotides. Therefore, it is likely to be assumed to have similar sensitivity to gamma rays as SARS-CoV.

 

SARS-CoV thermal inactivation:

In the case of SARS-CoV protein-free aqueous suspension samples, inactivation is possible within 30 minutes at 56 ° C. Unfortunately, for the sample containing the 20% protein suspension, residual virus activity was observed after the procedure. For fluids containing protein that may be contaminated with viruses, we recommend using a temperature of 60 ° C for 30 minutes. Literature data are not consistent, however, and some papers recommend using 70 ° C for a minimum of 45 minutes.

 

Impact of pH on SARS-CoV inactivation:

There are studies indicating that a strongly acidic or alkaline environment inactivates SARS-CoV. Undetectable virus activity in the sample occurs after an hour of incubation at pH 3 (or less) at room temperature. As the temperature drops to 4 ° C, the inactivation process slows down significantly. An hour of incubation in a strongly alkaline environment at pH 12 at room temperature leads to inactivation of SARS-CoV. At pH 13, half an hour is sufficient to achieve undetectable virus activity in the sample.

 

Inactivation of SARS-CoV by chemical agents:

Research results indicate that SARS-CoV is highly sensitive to chemical disinfectants. A 70% ethanol solution inactivates this virus within 1 minute. 6% acetic acid (1 minute), 0.1% sodium hypochlorite (1 minute) and 1% formaldechide (2 minutes) have similar effects. Interestingly, 0.23% pine oil showed the ability to inactivate SARS-CoV in 30 seconds.

http://www.e-manus.pl/